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"Detection of hybridization and species identification in domesticated and wild quails using genetic markers
Andreia J. AMARAL1*, Ana B. SILVA1, Ana R. GROSSO1, Lounès CHIKHI2, Cristiane BASTOS-
SILVEIRA3 and Deodália DIAS 1
1 Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Animal, Centro de Biologia Ambiental, Edifício C2-Piso 3, Campo Grande, 1749-016 Lisboa, Portugal
*Current address: Animal Breeding and Genomics Centre, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands; e-mail: andreia.fonseca@wur.nl
2 UMR CNRS 5174 “Evolution and Biologic Diversity”, Bât. 4R3 b2 – 118, Route de Narbonne, Université Paul Sabatier, 31062 Toulouse cédex 4, France
3 Universidade de Lisboa, Centro de Biologia Ambiental, Museu Nacional de Historia Natural, Rua da Politécnica no58, 1269-102 Lisboa, Portugal
Received 10 April 2007; Accepted 17 September 2007
Abstract. Hybridization is particularly widespread in birds and can affect species status and recovery. The common quail Coturnix coturnix is a protected game species that has undergone significant population decrease due to habitat changes. The release of Japanese quail C. japonica and or hybrids for restocking has been occurring since the 1970’s. Both species have not developed reproductive isolating mechanisms and hybridization is occurring. Species distinction based on morphology and male callings is difficult. In this work cytochrome b gene and five microsatellite loci were used with the aim of establishing an identification test for quails sampled in Portugal. Cytochrome b gene revealed to be of promising use to identify the quail maternal lineage. Success in species assignment with the studied microsatellite loci was moderate to identify samples with suspicion of being hybrids with common quail maternal lineage.
Key words: Coturnix coturnix, Coturnix japonica, microsatellites, cytochrome b, hybrids"
in Folia Zool. – 56(3): 285–300 (2007)
Andreia J. AMARAL1*, Ana B. SILVA1, Ana R. GROSSO1, Lounès CHIKHI2, Cristiane BASTOS-
SILVEIRA3 and Deodália DIAS 1
1 Universidade de Lisboa, Faculdade de Ciências, Departamento de Biologia Animal, Centro de Biologia Ambiental, Edifício C2-Piso 3, Campo Grande, 1749-016 Lisboa, Portugal
*Current address: Animal Breeding and Genomics Centre, Wageningen University, P.O. Box 338, 6700 AH Wageningen, The Netherlands; e-mail: andreia.fonseca@wur.nl
2 UMR CNRS 5174 “Evolution and Biologic Diversity”, Bât. 4R3 b2 – 118, Route de Narbonne, Université Paul Sabatier, 31062 Toulouse cédex 4, France
3 Universidade de Lisboa, Centro de Biologia Ambiental, Museu Nacional de Historia Natural, Rua da Politécnica no58, 1269-102 Lisboa, Portugal
Received 10 April 2007; Accepted 17 September 2007
Abstract. Hybridization is particularly widespread in birds and can affect species status and recovery. The common quail Coturnix coturnix is a protected game species that has undergone significant population decrease due to habitat changes. The release of Japanese quail C. japonica and or hybrids for restocking has been occurring since the 1970’s. Both species have not developed reproductive isolating mechanisms and hybridization is occurring. Species distinction based on morphology and male callings is difficult. In this work cytochrome b gene and five microsatellite loci were used with the aim of establishing an identification test for quails sampled in Portugal. Cytochrome b gene revealed to be of promising use to identify the quail maternal lineage. Success in species assignment with the studied microsatellite loci was moderate to identify samples with suspicion of being hybrids with common quail maternal lineage.
Key words: Coturnix coturnix, Coturnix japonica, microsatellites, cytochrome b, hybrids"
in Folia Zool. – 56(3): 285–300 (2007)
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